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Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
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Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
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May 20, 2015
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To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.
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Choi, T., Khaliq, M., Ko, S., So, J., Shin, D. Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration. J. Vis. Exp. (99), e52785, doi:10.3791/52785 (2015).
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