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Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
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Developmental Biology
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JoVE Journal Developmental Biology
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

DOI:
10.3791/52785-v

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08:14 min

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May 20, 2015

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, , , ,
1Department of Developmental Biology, McGowan Institute for Regenerative Medicine,University of Pittsburgh

Chapters

  • 00:05Title
  • 01:30Embryo/larva Preparation
  • 02:42Metronidazole (Mtz) Treatment and Biliary-driven Liver Regeneration Analysis
  • 06:07Results: Representative Liver Ablation and Regeneration Analysis
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.

Tags

Hepatocyte-specific AblationBiliary-driven Liver RegenerationZebrafish ModelPharmacogenetic Hepatocyte AblationBEC-derived HepatocytesLiver Regeneration MechanismsChemical Screening

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