/
/
Экс Utero электропорации и Органотипической фрагмент Культура мыши ткани гиппокампа
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Экс Utero электропорации и Органотипической фрагмент Культура мыши ткани гиппокампа
9,356 Views
•
09:17 min
•
March 04, 2015
DOI:
Özet
Automatically generated
Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.
Read Article
Cite this Article
Venkataramanappa, S., Simon, R., Britsch, S. Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue. J. Vis. Exp. (97), e52550, doi:10.3791/52550 (2015).
Copy