/
/
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
9,360 Views
•
09:17 min
•
March 04, 2015
DOI:
Summary
Automatically generated
Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.
Read Article
Cite this Article
Venkataramanappa, S., Simon, R., Britsch, S. Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue. J. Vis. Exp. (97), e52550, doi:10.3791/52550 (2015).
Copy