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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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March 04, 2015
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Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.
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Venkataramanappa, S., Simon, R., Britsch, S. Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue. J. Vis. Exp. (97), e52550, doi:10.3791/52550 (2015).
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