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小鼠海马组织的前子宫内电和器官切片文化
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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小鼠海马组织的前子宫内电和器官切片文化

9,356 Views

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09:17 min

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March 04, 2015

DOI:

10.3791/52550-v

DOI:
10.3791/52550-v

•

09:17 min
March 04, 2015

•

2 Views
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1Institute of Molecular and Cellular Anatomy,University of Ulm

Özet

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Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.

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Venkataramanappa, S., Simon, R., Britsch, S. Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue. J. Vis. Exp. (97), e52550, doi:10.3791/52550 (2015).

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