The question of how chromatin regulators and chromatin states affect the genome in vivo is key to our understanding of how early cell fate decisions are made in the developing embryo. ChIP-Seq—the most popular approach to investigate chromatin features at a global level—is outlined here for Xenopus embryos.
Gentsch, G. E., Patrushev, I., Smith, J. C. Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq. J. Vis. Exp. (96), e52535, doi:10.3791/52535 (2015).