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发育生物学
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Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

DOI:
10.3791/52535-v

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10:23 min

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February 26, 2015

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, ,
1Division of Systems Biology,MRC National Institute for Medical Research

Chapters

  • 00:05Title
  • 01:37Chromatin Cross-linking
  • 02:55Chromatin Extraction
  • 04:31Chromatin Fragmentation
  • 06:07Chromatin Immunoprecipitation
  • 08:16Results: Chromatin Binding of the T-box Transcription Factor VegT at Gastrula Stage of X. tropicalis Embryos
  • 09:54Conclusion

Summary

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The question of how chromatin regulators and chromatin states affect the genome in vivo is key to our understanding of how early cell fate decisions are made in the developing embryo. ChIP-Seq—the most popular approach to investigate chromatin features at a global level—is outlined here for Xenopus embryos.

Tags

Chromatin RegulatorsChromatin StatesXenopus EmbryosChIP-SeqChromatin ImmunoprecipitationGenome-wide ProfilingDNA-binding ProteinsTranscription FactorsChromatin ModifiersHistone ModificationsEmbryogenesisModel OrganismsGenome Assembly

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