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Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
JoVE Journal
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JoVE Journal Kimya
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

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07:54 min

March 12, 2015

DOI:

07:54 min
March 12, 2015

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Özet

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Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

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