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Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
JoVE 杂志
化学
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JoVE 杂志 化学
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
DOI:

07:54 min

March 12, 2015

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Chapters

  • 00:05Title
  • 01:06Preparing Plates with Affixed Sterile Windows
  • 02:38Fixing the Cells
  • 04:48Sample Mounting and Imaging
  • 06:33Images after Successful and Unsuccessful Sample Processing
  • 07:14Conclusion

Summary

自动翻译

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

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