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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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February 07, 2015
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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
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Bharadwaj, M. S., Tyrrell, D. J., Lyles, M. F., Demons, J. L., Rogers, G. W., Molina, A. J. A. Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy. J. Vis. Exp. (96), e52350, doi:10.3791/52350 (2015).
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