ジャーナル
/
医学
/
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
JoVE Journal
医学
This content is Free Access.
JoVE Journal 医学
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

DOI:
10.3791/52350-v

•

11:27 min

•

February 07, 2015

•

, , , , ,
1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine,Wake Forest School of Medicine, 2Seahorse Biosciences

章

  • 00:05標題
  • 01:38Skeletal Muscle Biopsy
  • 03:20Mitochondrial Isolation and Washing
  • 06:35Perform Extracellular Flux (XF) Assays
  • 08:32Results: Respirometric Assessment of Mitochondria
  • 10:11Conclusion

概要

自動翻訳

自動翻訳

Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

タグ

MitochondriaSkeletal MusclePercutaneous Needle BiopsyRespirometryExtracellular Flux AnalysisElectron Transport ChainCOX IVGAPDH

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Myo-mechanical Analysis of Isolated Skeletal Muscle

Skeletal Muscle Gender Dimorphism from Proteomics

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique

Substernal Thyroid Biopsy Using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Povidone Iodine Rectal Preparation at Time of Prostate Needle Biopsy is a Simple and Reproducible Means to Reduce Risk of Procedural Infection

Read Article