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The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
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The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

21,152 Views

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12:18 min

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December 26, 2014

DOI:

10.3791/52220-v

DOI:
10.3791/52220-v

•

12:18 min
December 26, 2014

•

16 Views
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1Physiology and Bosch Institute,University of Sydney, 2Motor Neuron Disease Research Group, Australian School of Advanced Medicine,Macquarie University, 3Advanced Microscopy Facility, Bosch Institute,University of Sydney

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The neuromuscular junction (NMJ) is altered in a variety of conditions that can sometimes culminate in synaptic failure. This report describes fluorescence microscope-based methods to quantify such structural changes.

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Tse, N., Morsch, M., Ghazanfari, N., Cole, L., Visvanathan, A., Leamey, C., Phillips, W. D. The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy. J. Vis. Exp. (94), e52220, doi:10.3791/52220 (2014).

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