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Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
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JoVE Journal İmmünoloji ve Enfeksiyon
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

DOI:
10.3791/52140-v

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10:03 min

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December 12, 2014

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,
1Inserm U1016,Institut Cochin, 2Cnrs, UMR8104, 3Université Paris Descartes,Sorbonne Paris Cité

Bölümler

  • 00:05Başlık
  • 01:32Chemokine Stimulation
  • 02:41Cell Staining
  • 04:14Image Acquisition and Analysis
  • 07:23Results: Representative Chemokine-induced T Cell Polarization
  • 09:30Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

Etiketler

Cell PolarizationChemokine SignalingImaging Flow CytometryActin CytoskeletonMHC Class IPhospho-ERMPar6/aPKC PathwayPharmacological Inhibition

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