Journal
/
Immunologie und Infektion
/
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

DOI:
10.3791/52140-v

•

10:03 min

•

December 12, 2014

•

,
1Inserm U1016,Institut Cochin, 2Cnrs, UMR8104, 3Université Paris Descartes,Sorbonne Paris Cité

Kapitel

  • 00:05Titel
  • 01:32Chemokine Stimulation
  • 02:41Cell Staining
  • 04:14Image Acquisition and Analysis
  • 07:23Results: Representative Chemokine-induced T Cell Polarization
  • 09:30Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

Tags

Cell PolarizationChemokine SignalingImaging Flow CytometryActin CytoskeletonMHC Class IPhospho-ERMPar6/aPKC PathwayPharmacological Inhibition

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Read Article