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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

94,358 Views

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09:40 min

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January 03, 2015

DOI:

10.3791/52118-v

DOI:
10.3791/52118-v

•

09:40 min
January 03, 2015

•

22 Views
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1Harvard Tıp Fakültesi, 2Division of Hematology/Oncology,Boston Children’s Hospital, 3Department of Pediatric Oncology,Dana-Farber Cancer Institute, 4Howard Hughes Medical Institute

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CRISPR/Cas9 is a robust system to produce disruption of genes and genetic elements. Here we describe a protocol for the efficient creation of genomic deletions in mammalian cell lines using CRISPR/Cas9.

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Genomic DeletionsCRISPR/Cas9Genome EngineeringSingle Guide RNA (sgRNA)Double-strand Breaks (DSBs)Non-homologous End Joining (NHEJ)Loss-of-function StudiesMammalian Cell Lines

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Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).

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