Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Daniel E. Bauer; Matthew C. Canver; Stuart H. Orkin

doi:10.3791/52118

Journal

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生物学

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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

94,358 Views

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09:40 min

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January 03, 2015

DOI:

10.3791/52118-v

DOI:

10.3791/52118-v

•

09:40 min

January 03, 2015

•

22 Views

Daniel E. Bauer*^1,2,3, Matthew C. Canver*¹, Stuart H. Orkin^1,2,3,4

¹Harvard Medical School, ²Division of Hematology/Oncology,Boston Children’s Hospital, ³Department of Pediatric Oncology,Dana-Farber Cancer Institute, ⁴Howard Hughes Medical Institute

Summary

CRISPR/Cas9 is a robust system to produce disruption of genes and genetic elements. Here we describe a protocol for the efficient creation of genomic deletions in mammalian cell lines using CRISPR/Cas9.

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Genomic Deletions CRISPR/Cas9 Genome Engineering Single Guide RNA (sgRNA)Double-strand Breaks (DSBs)Non-homologous End Joining (NHEJ)Loss-of-function Studies Mammalian Cell Lines

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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Summary

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