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Long-term Time Lapse Imaging of Mouse Cochlear Explants
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JoVE Journal Nörobilim
Long-term Time Lapse Imaging of Mouse Cochlear Explants

Long-term Time Lapse Imaging of Mouse Cochlear Explants

9,394 Views

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10:43 min

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November 02, 2014

DOI:

10.3791/52101-v

DOI:
10.3791/52101-v

•

10:43 min
November 02, 2014

•

6 Views
,
1Biological Sciences Platform,Sunnybrook Research Institute, 2Department of Otolaryngology – Head and Neck Surgery,University of Toronto, 3Department of Laboratory Medicine and Pathobiology,University of Toronto

Özet

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Live imaging of the embryonic mammalian cochlea is challenging because the developmental processes at hand operate on a temporal gradient over ten days. Here we present a method for culturing and then imaging embryonic cochlear explant tissue taken from a fluorescent reporter mouse over five days.

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Cite this Article

Mulvaney, J. F., Dabdoub, A. Long-term Time Lapse Imaging of Mouse Cochlear Explants. J. Vis. Exp. (93), e52101, doi:10.3791/52101 (2014).

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