需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Long-term Time Lapse Imaging of Mouse Cochlear Explants
Chapters
- 00:05Title
- 01:31Culturing Embryonic Cochleae and Explants
- 05:56Live Imaging
- 08:57Results: Cochlear Explant Time Lapse
- 10:06Conclusion
Live imaging of the embryonic mammalian cochlea is challenging because the developmental processes at hand operate on a temporal gradient over ten days. Here we present a method for culturing and then imaging embryonic cochlear explant tissue taken from a fluorescent reporter mouse over five days.
