Dergi
/
/
Isolation of Murine Lymph Node Stromal Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

30,634 Views

05:47 min

August 19, 2014

DOI:

05:47 min
August 19, 2014

18 Views
, , ,

Özet

Automatically generated

Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

Read Article