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Isolation of Murine Lymph Node Stromal Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

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05:47 min

August 19, 2014

DOI:

05:47 min
August 19, 2014

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Özet

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Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

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