Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Isolation of Murine Lymph Node Stromal Cells
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Immunology and Infection
Isolation of Murine Lymph Node Stromal Cells
 

Isolation of Murine Lymph Node Stromal Cells

Article DOI: 10.3791/51803-v • 05:47 min • August 19th, 2014
August 19th, 2014 •
*1, *1, 1, 1
1Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

Tags

Isolation Murine Lymph Node Stromal Cells Secondary Lymphoid Organs Stromal Cell Subsets CD31 Podoplanin (gp38) T Zone Reticular Cells Fibroblastic Reticular Cells Lymphatic Endothelial Cells Blood Endothelial Cells FRC-like Pericytes Double Negative Cell Population Functions Of Stromal Cells Attraction And Interaction With Different Cell Types Filtration Of Draining Fluidics Contraction Of Lymphatic Vessels Role Of Stromal Cells In Regulating Cytotoxic T Cell Responses Appropriate Procedure For Isolation Of Desired Cell Populations Enzymatic Digestion Incubation Times Loss Of Surface Marker Expression Cell Death
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter