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Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
JoVE Journal
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JoVE Journal Davranış
Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

11,462 Views

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08:14 min

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August 26, 2014

DOI:

10.3791/51642-v

DOI:
10.3791/51642-v

•

08:14 min
August 26, 2014

•

1 Views
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1The Alexander Silberman Institute of Life Sciences & Edmond and Lily Safra Center for Brain Sciences,Kudüs İbrani Üniversitesi

Özet

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This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

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Transcription DynamicsBrain SamplesBehavioral ExperienceGene ExpressionMolecular NeuroscienceNeuropsychiatric DisordersTranscriptional ProgramsNucleus AccumbensCocaine ExperienceRNA PurificationReverse TranscriptionMicrofluidic ArraysQPCR AnalysisSingle CellsWhole-genome StudiesMicroarraysRNAseq

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Turm, H., Mukherjee, D., Haritan, D., Tahor, M., Citri, A. Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience. J. Vis. Exp. (90), e51642, doi:10.3791/51642 (2014).

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