Journal
/
行为学
/
Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
JoVE 杂志
行为学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 行为学
Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

11,471 Views

•

08:14 min

•

August 26, 2014

DOI:

10.3791/51642-v

DOI:
10.3791/51642-v

•

08:14 min
August 26, 2014

•

1 Views
, , , ,
1The Alexander Silberman Institute of Life Sciences & Edmond and Lily Safra Center for Brain Sciences,The Hebrew University of Jerusalem

Summary

Automatically generated

This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

Explore More Videos

Transcription DynamicsBrain SamplesBehavioral ExperienceGene ExpressionMolecular NeuroscienceNeuropsychiatric DisordersTranscriptional ProgramsNucleus AccumbensCocaine ExperienceRNA PurificationReverse TranscriptionMicrofluidic ArraysQPCR AnalysisSingle CellsWhole-genome StudiesMicroarraysRNAseq

Read Article

Cite this Article

Turm, H., Mukherjee, D., Haritan, D., Tahor, M., Citri, A. Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience. J. Vis. Exp. (90), e51642, doi:10.3791/51642 (2014).

Download .ris file

Copy

Share Video

.

Copy