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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em
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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em

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07:35 min

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June 09, 2014

DOI:

10.3791/51583-v

DOI:
10.3791/51583-v

•

07:35 min
June 09, 2014

•

11 Views
, ,
1Department of Biomedical Engineering,Duke University, 2Research Triangle MRSEC,Duke University

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弹性蛋白样多肽是刺激 - 反应的生物聚合物与应用，从重组蛋白纯化给药。这个协议描述了来自大肠杆菌的使用其低临界溶解温度的相变行为作为一个简单的替代色谱弹性蛋白样多肽和其肽或蛋白质融合的纯化和表征。

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Cite this Article

MacEwan, S. R., Hassouneh, W., Chilkoti, A. Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli. J. Vis. Exp. (88), e51583, doi:10.3791/51583 (2014).

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