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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
JoVE 杂志
生物学
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JoVE 杂志 生物学
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

21,087 Views

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07:35 min

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June 09, 2014

DOI:

10.3791/51583-v

DOI:
10.3791/51583-v

•

07:35 min
June 09, 2014

•

4 Views
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1Department of Biomedical Engineering,Duke University, 2Research Triangle MRSEC,Duke University

Tags

Elastin-like PolypeptidesRecombinantNon-chromatographic PurificationLower Critical Solution TemperaturePhase TransitionCoacervationEscherichia ColiPeptide FusionProtein FusionBiosensingDrug DeliveryThermal Properties

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MacEwan, S. R., Hassouneh, W., Chilkoti, A. Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli. J. Vis. Exp. (88), e51583, doi:10.3791/51583 (2014).

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