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Journal / Biology / Non-chromatographic Purification of Recombinant…
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Biology

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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
 

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Article DOI: 10.3791/51583-v • 07:35 min • June 9th, 2014
June 9th, 2014 •
*1,2, *1,2, 1,2
1Department of Biomedical Engineering, Duke University, 2Research Triangle MRSEC, Duke University
* These authors contributed equally

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Elastin-like polypeptides are stimulus-responsive biopolymers with applications ranging from recombinant protein purification to drug delivery. This protocol describes the purification and characterization of elastin-like polypeptides and their peptide or protein fusions from Escherichia coli using their lower critical solution temperature phase transition behavior as a simple alternative to chromatography.

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Keywords: Elastin-like Polypeptides Recombinant Non-chromatographic Purification Lower Critical Solution Temperature Phase Transition Coacervation Escherichia Coli Peptide Fusion Protein Fusion Biosensing Tissue Engineering Drug Delivery Thermal Properties
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