Pregnancy leads to significant changes to the fatty acid composition of maternal tissues. Lipid profiles can be obtained via gas chromatography to allow identification and quantification of fatty acids in individual lipid classes among rats fed various high and low fat diets during pregnancy.
Fisk, H. L., West, A. L., Childs, C. E., Burdge, G. C., Calder, P. C. The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy. J. Vis. Exp. (85), e51445, doi:10.3791/51445 (2014).