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Chapters
- 00:05Title
- 01:28Preparation of a Total Lipid Extract
- 03:00Separation of Lipid Classes by Solid Phase Extraction
- 05:19Preparation of Fatty Acid Methyl Esters from Cholesteryl Esters
- 06:26Removal of Cholesterol Contamination and Gas Chromatographic Analysis
- 08:02Results: Identification and Quantification of Fatty Acids in Rat Liver Tissue by Gas Chromatography
- 10:24Conclusion
Pregnancy leads to significant changes to the fatty acid composition of maternal tissues. Lipid profiles can be obtained via gas chromatography to allow identification and quantification of fatty acids in individual lipid classes among rats fed various high and low fat diets during pregnancy.
