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Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

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09:07 min

September 19, 2013

DOI:

09:07 min
September 19, 2013

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Özet

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This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.

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