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Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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September 19, 2013
DOI:
1Department of General Neurology,Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology,University of Bern, 3Department of Immunology,University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery,University Clinic Tuebingen, 5Department of Neurology,University Hospital Erlangen
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This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
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Stoeckle, C., Rota, I. A., Tolosa, E., Haller, C., Melms, A., Adamopoulou, E. Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus. J. Vis. Exp. (79), e50951, doi:10.3791/50951 (2013).
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