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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
Bölümler
- 00:05Başlık
- 01:26Preparation of Compound Dilution Series
- 02:55Plate Preparation and Spotting
- 04:44Substrate Addition and Fluorescence Measurement
- 05:32Automated Operation for High-throughput Screening
- 07:30Results: Analyzing the FRET-based Botulinum Neurotoxin/A Light Chain Assay Kinetic Data
- 09:39Conclusion
The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.
