Journal
/
Chemie
/
A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
JoVE Journal
Chemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Chemie
A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

DOI:
10.3791/50908-v

•

10:30 min

•

December 27, 2013

•

, , , ,
1Department of Chemistry,The Scripps Research Institute, 2Worm Institute of Research and Medicine (WIRM),The Scripps Research Institute

Kapitel

  • 00:05Titel
  • 01:26Preparation of Compound Dilution Series
  • 02:55Plate Preparation and Spotting
  • 04:44Substrate Addition and Fluorescence Measurement
  • 05:32Automated Operation for High-throughput Screening
  • 07:30Results: Analyzing the FRET-based Botulinum Neurotoxin/A Light Chain Assay Kinetic Data
  • 09:39Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.

Tags

Botulinum NeurotoxinBoNTLight ChainFRETHigh-throughput ScreeningHTSInhibitorAcetylcholineNeurotransmitter ReleaseTherapeutic TargetSmall Molecule

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Synthesis and Characterization of Functionalized Metal-organic Frameworks

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

Ethylene Polymerizations Using Parallel Pressure Reactors and a Kinetic Analysis of Chain Transfer Polymerization

Atom Transfer Radical Polymerization of Functionalized Vinyl Monomers Using Perylene as a Visible Light Photocatalyst

Synthesis and Characterization of Supramolecular Colloids

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Preparation and Characterization of C60/Graphene Hybrid Nanostructures

Synthesis of a Thiol Building Block for the Crystallization of a Semiconducting Gyroidal Metal-sulfur Framework

Read Article