We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.
Verchère, A., Dezi, M., Broutin, I., Picard, M. In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa. J. Vis. Exp. (84), e50894, doi:10.3791/50894 (2014).