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In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
JoVE Journal
生物学
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JoVE Journal 生物学
In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

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13:40 min

February 17, 2014

DOI:

13:40 min
February 17, 2014

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概要

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We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.

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