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FtsZ Polymerization Assays: Simple Protocols and Considerations
Bölümler
- 00:05Başlık
- 01:20FtsZ Sedimentation Assay at Slow Spin
- 03:2990° Light Scattering
- 05:19GTP Hydrolysis Assay
- 09:33Results: Buffer Influence on Tubule Formation
- 11:40Conclusion
Polymerization of FtsZ is essential for bacterial cell division. In this report, we detail simple protocols to monitor FtsZ polymerization activity and discuss the influence of buffer composition. The protocols can be used to study the interaction of FtsZ with regulatory proteins or antibacterial drugs that affect FtsZ polymerization.
