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FtsZ Polymerization Assays: Simple Protocols and Considerations
JoVE Journal
生物学
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JoVE Journal 生物学
FtsZ Polymerization Assays: Simple Protocols and Considerations

FtsZ Polymerization Assays: Simple Protocols and Considerations

15,009 Views

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12:04 min

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November 16, 2013

DOI:

10.3791/50844-v

DOI:
10.3791/50844-v

•

12:04 min
November 16, 2013

•

3 Views
,
1Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute,University of Groningen

概要

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Polymerization of FtsZ is essential for bacterial cell division. In this report, we detail simple protocols to monitor FtsZ polymerization activity and discuss the influence of buffer composition. The protocols can be used to study the interaction of FtsZ with regulatory proteins or antibacterial drugs that affect FtsZ polymerization.

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Cite this Article

Król, E., Scheffers, D. FtsZ Polymerization Assays: Simple Protocols and Considerations. J. Vis. Exp. (81), e50844, doi:10.3791/50844 (2013).

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