This paper describes the application of cryoanalytical electron microscopy to the quantitative measurement of total calcium content and distribution at subcellular resolution in physiologically defined biological specimens.
Pivovarova, N. B., Andrews, S. B. Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis. J. Vis. Exp. (81), e50807, doi:10.3791/50807 (2013).