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Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
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神经科学
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JoVE 杂志 神经科学
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

11,917 Views

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11:42 min

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November 20, 2013

DOI:

10.3791/50807-v

DOI:
10.3791/50807-v

•

11:42 min
November 20, 2013

•

9 Views
,
1Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Summary

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This paper describes the application of cryoanalytical electron microscopy to the quantitative measurement of total calcium content and distribution at subcellular resolution in physiologically defined biological specimens.

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Cite this Article

Pivovarova, N. B., Andrews, S. B. Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis. J. Vis. Exp. (81), e50807, doi:10.3791/50807 (2013).

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