Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
Bölümler
- 00:05Başlık
- 01:29Injection of PRV into Mice
- 04:04Cranial Injection of Biotinylated Dextran Amines
- 06:41Detection of BDA and CTb in the Same Sections
- 09:08PRV-infected Neurons and BDA and CTb Circuit Confirmation
- 12:31Conclusion
Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.
