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Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
章
- 00:05標題
- 01:29Injection of PRV into Mice
- 04:04Cranial Injection of Biotinylated Dextran Amines
- 06:41Detection of BDA and CTb in the Same Sections
- 09:08PRV-infected Neurons and BDA and CTb Circuit Confirmation
- 12:31Conclusion
Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.
