Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice
13,605 Views
•
12:07 min
•
August 17, 2012
DOI:
Özet
Automatically generated
An optimized procedure to purify neural crest-derived neuronal progenitors from fetal mouse tissues is described. This method takes advantage of expression from fluorescent reporter alleles to isolate discrete populations by fluorescence-activated cell sorting (FACS). The technique can be applied to isolate neuronal subpopulations throughout development or from adult tissues.
Explore More Videos
Fluorescence-activated Cell SortingFACSAutonomic Neural ProgenitorsVisceral OrgansFetal MiceNeural CrestAutonomic GangliaLaser Capture Micro-dissectionFluorescent ReportersTyrosine HydroxylaseTH GeneCatecholaminesEnteric Neuronal ProgenitorsMigrationFetal IntestinePelvic Ganglia NeuronsEGFPTransgene Construct
Read Article
Cite this Article
Buehler, D. P., Wiese, C. B., Skelton, S. B., Southard-Smith, E. M. An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice. J. Vis. Exp. (66), e4188, doi:10.3791/4188 (2012).
Copy