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An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice

An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice

DOI:
10.3791/4188-v

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12:07 min

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August 17, 2012

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, , ,
1Division of Genetic Medicine, Department of Medicine,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 02:05Dissection
  • 04:34Dissociation of Subdissected Tissues
  • 05:59Filtering Cell Suspension
  • 07:04Preparing Samples for FACS
  • 08:00Flow Cytometry
  • 08:47Results: Purification of Neural Crest-derived Neuronal Progenitors from Fetal Mouse Tissues
  • 11:32Conclusion

Summary

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自动翻译

An optimized procedure to purify neural crest-derived neuronal progenitors from fetal mouse tissues is described. This method takes advantage of expression from fluorescent reporter alleles to isolate discrete populations by fluorescence-activated cell sorting (FACS). The technique can be applied to isolate neuronal subpopulations throughout development or from adult tissues.

Tags

Fluorescence-activated Cell SortingFACSAutonomic Neural ProgenitorsVisceral OrgansFetal MiceNeural CrestAutonomic GangliaLaser Capture Micro-dissectionFluorescent ReportersTyrosine HydroxylaseTH GeneCatecholaminesEnteric Neuronal ProgenitorsMigrationFetal IntestinePelvic Ganglia NeuronsEGFPTransgene Construct

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