Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
13,847 Views
•
22:02 min
•
June 30, 2012
DOI:
Özet
Automatically generated
The mouse inner ear is a placode-derived sensory organ whose developmental program is elaborated during gestation. We define an in utero gene transfer technique consisting of three steps: mouse ventral laparotomy, transuterine microinjection, and in vivo electroporation. We use digital video microscopy to demonstrate the critical experimental embryological techniques.
Explore More Videos
Gene TransferDeveloping Mouse Inner EarIn Vivo ElectroporationSensory EpitheliaCristaeMaculaeOrgan Of CortiVestibular Hair CellsAuditory Hair CellsCell Fate SpecificationMorphogenesisGestationTransgenic EmbryosMolecular BasisGain-of-function StudiesLoss-of-function StudiesGene Misexpression StudiesVentral LaparotomyTransuterine Microinjection
Read Article
Cite this Article
Wang, L., Jiang, H., Brigande, J. V. Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation. J. Vis. Exp. (64), e3653, doi:10.3791/3653 (2012).
Copy