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Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
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JoVE Journal İmmünoloji ve Enfeksiyon
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

12,616 Views

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11:18 min

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May 07, 2012

DOI:

10.3791/3627-v

DOI:
10.3791/3627-v

•

11:18 min
May 07, 2012

•

1 Views
, , ,
1Center for Cell and Gene Therapy,Baylor Tıp Fakültesi , 2Pathology and Immunology,Baylor Tıp Fakültesi , 3Department of Stem Cell Transplantation and Cellular Therapy,University of Texas M.D. Anderson Cancer Center, 4Tıp,Baylor Tıp Fakültesi , 5Pediatri Anabilim Dalı,Baylor Tıp Fakültesi

Özet

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Here we describe the first good manufacturing practice (GMP)-compliant method of producing virus-specific cytotoxic T lymphocytes (CTL) from umbilical cord blood, a source of predominantly naîve T cells.

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Cytotoxic T LymphocytesUmbilical Cord BloodCytomegalovirusEpstein-Barr VirusAdenovirusStem Cell TransplantationCord Blood TransplantationVirus-specific CTLDendritic CellsAdenoviral VectorCMV Antigen Pp65IL-7IL-12IL-15EBV-transformed B CellsEBV-LCL

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Hanley, P. J., Lam, S., Shpall, E. J., Bollard, C. M. Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus. J. Vis. Exp. (63), e3627, doi:10.3791/3627 (2012).

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