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A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium
JoVE Journal
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A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

DOI:
10.3791/3394-v

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05:19 min

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January 16, 2012

•

,
1Biosciences, College of Life and Environmental Sciences,The University of Exeter

Bölümler

  • 00:05Başlık
  • 01:54Mounting Leaf Samples in PFD
  • 03:20Representative Leaf Imaging Results
  • 04:37Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe the use of perfluorodecalin as an infiltrative mounting medium. This is a simple method for improving depth of imaging in Arabidopsis thaliana leaf tissue with minimal physiological impact.

Etiketler

Arabidopsis LeavesImagingPerfluorodecalinInfiltrative Imaging MediumHigh-resolution ImagesRefractive IndexBiological SamplesSpongy MesophyllPlant LeavesVertebrate LungsFluorescence MicroscopyPerfluorocarbonLaser Scanning Confocal MicroscopyPFD ProtocolOptical ComplexityWater Refractive IndexCytosol Refractive IndexNon-fluorescentNon-toxicSurface Tension

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