We describe the use of perfluorodecalin as an infiltrative mounting medium. This is a simple method for improving depth of imaging in Arabidopsis thaliana leaf tissue with minimal physiological impact.
Littlejohn, G. R., Love, J. A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium. J. Vis. Exp. (59), e3394, doi:10.3791/3394 (2012).