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In vivo Dual Substrate Bioluminescent Imaging
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October 11, 2011
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Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
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In VivoDual Substrate Bioluminescent ImagingBreast Cancer CellsPrimary TumorsMetastatic SitesIn Vivo Bioluminescent Imaging TechnologiesTumor GrowthLongitudinal StudyResearchersBreast Cancer MetastasisCell Signaling SystemsGene ExpressionTumor DevelopmentMetastatic ProgressionLuciferase EnzymesFirefly LuciferaseSea Pansy Luciferase
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Wendt, M. K., Molter, J., Flask, C. A., Schiemann, W. P. In vivo Dual Substrate Bioluminescent Imaging. J. Vis. Exp. (56), e3245, doi:10.3791/3245 (2011).
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