Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
Wendt, M. K., Molter, J., Flask, C. A., Schiemann, W. P. In vivo Dual Substrate Bioluminescent Imaging. J. Vis. Exp. (56), e3245, doi:10.3791/3245 (2011).