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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

DOI:
10.3791/3084-v

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07:26 min

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May 01, 2011

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, , , ,
1Center for Biological Security,Robert Koch-Institute, 2CICVyA – INTA Castelar,Instituto de Virología, 3Center of Molecular Immunology,Ciudad de la Habana, Cuba, 4Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology,Free University of Berlin, 5Institut of Pharmacology,Charité-University Medicine of Berlin

Bölümler

  • 00:05Başlık
  • 01:19IgY-extraction by Means of PEG-precipitation
  • 06:53Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.

Etiketler

IgY TechnologyChicken AntibodiesEgg YolkPolyethylene Glycol (PEG) PrecipitationImmunizationI.m. VaccinationGene-Gun Plasmid-immunizationAntibody-titresBoost ImmunizationsLaying CapacityExtraction Of Total IgYPrecipitation ProcedureRemoval Of LipidsDialysisIgY-extract StoragePurity Of The ExtractTotal IgY Per EggAge Of The Laying HenTotal Potential Harvest Of IgY

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