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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
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免疫学と感染
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JoVE Journal 免疫学と感染
IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

DOI:
10.3791/3084-v

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07:26 min

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May 01, 2011

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, , , ,
1Center for Biological Security,Robert Koch-Institute, 2CICVyA – INTA Castelar,Instituto de Virología, 3Center of Molecular Immunology,Ciudad de la Habana, Cuba, 4Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology,Free University of Berlin, 5Institut of Pharmacology,Charité-University Medicine of Berlin

章

  • 00:05標題
  • 01:19IgY-extraction by Means of PEG-precipitation
  • 06:53Conclusion

概要

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自動翻訳

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.

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IgY TechnologyChicken AntibodiesEgg YolkPolyethylene Glycol (PEG) PrecipitationImmunizationI.m. VaccinationGene-Gun Plasmid-immunizationAntibody-titresBoost ImmunizationsLaying CapacityExtraction Of Total IgYPrecipitation ProcedureRemoval Of LipidsDialysisIgY-extract StoragePurity Of The ExtractTotal IgY Per EggAge Of The Laying HenTotal Potential Harvest Of IgY

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